- Assay Type:Competitive
- Primary antibody reactivity:Human
- Description:Cotinine ELISA Kit
- Size:1 kit
- Detection Method:Colorimetric
- Detection Range:5 - 100 ng/ml
- Storage Temperature:4 °C
- Detection Limit:5 ng/ml
- Sample Volume:10 µL
- Regulatory Status:RUO
- Cat. No.:10630-348
- Supplier no.:KA0930
Cotinine ELISA kit is used for the measurement of Cotinine in serum and urine.
- Solid phase, competitive ELISA
- Cotinine is a reliable marker for smoking cessation studies
- Detection of Cotinine in serum and urine
Exposure to tobacco smoke can be detected by measuring nicotine and its metabolites
The Cotinine ELISA Kit is a solid phase competitive ELISA. The samples and Cotinine enzyme conjugate are added to the wells coated with anti-Cotinine antibody. Cotinine in the samples competes with a Cotinine enzyme (HRP) conjugate for binding sites. Unbound Cotinine and Cotinine enzyme conjugate is washed off by washing step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cotinine in the samples. A standard curve is prepared relating color intensity to the concentration of the Cotinine.
Nicotine has a short half-life and is not used as a marker for tobacco smoke exposure. Cotinine due to its longer half-life has been used in research as a reliable marker for smoking status and smoking cessation studies. The Cotinine ELISA Kit is designed for the detection Cotinine in serum and urine. It can also be adapted for other fluids.
); Cotinine HRP enzyme conjugate (12 mL
); TMB substrate (12 mL
) and Stop solution (12 mL
).