TransforMax™ EC100D™ pir+ and pir-116 Electrocompetent E. coli, Competent Cells, Biosearch Technologies

: Lucigen

TransforMax™ EC100D™ pir+ and pir-116 Electrocompetent E. coli, Competent Cells, Biosearch Technologies

: Lucigen

Rescue cloning of EZ-Tn5™ transposed elements.

Propagate plasmids or circular DNA tagged with the R6Kɣ origin of replication
Rescue transposition mutants created using the EZ-Tn5™ Insertion Kit or Tnp Transposome™ Kit
Control copy number of plasmids containing the R6Kɣ origin (15 copies per cell in EC100D pir+ cells; 250 copies per cell in EC100D pir-116 cells)
Stabilize large rescue clones for unbiased propagation

The TransforMax™ EC100D™ pir+ Electrocompetent E

coli and TransforMax™ EC100D™ pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγori). The cells are derived from Epicentre's TransforMax™ EC100™ Electrocompetent E. coli by P1 phage transduction with a strain containing the pir+ or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker. For cloning potentially toxic or unstable DNA sequences, choose TransforMax EC100D pir+ cells to maintain R6Kγori containing plasmids at approximately 15 copies per cell. Choose Max EC100D pir-116 cells for high-copy propagation up to 250 plasmid copies per cell.

EC100D competent cell strains are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. They are also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.

Genotypes:
TransforMax EC100D pir+ Electrocompetent E. coli:
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir+(DHFR)

Transformation Efficiency:
Greater than 5 x 10⁹ colony forming units (cfu)/µg with pR6Kan control DNA.

: Each kit contains 5 tubes with 100 µL of electrocompetent cells, which is enough cells for 10 electroporations. A control vector containing and R6K?ori is also included with each product.

: For research use only. Not for human or diagnostic use.

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