TransforMax™EC100™ Electrocompetent and Chemically Competent E. coli, Biosearch Technologies

Supplier: Lucigen

Certificates

Supplier: Lucigen

About this item

Clone large plasmids with high efficiency.

Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA
Select convenience with chemically competent format: >5 x 10⁸ cfu/µg pUC19 DNA
Choose electrocompetent format for higher efficiencies: >1 x 10¹⁰ cfu/µg pUC19 DNA
Ideal strain for general cloning, subcloning and plasmid preparation due to endA and recA mutation

The highly versatile TransforMax™ EC100™ E

EC100 competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. They are also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG.

coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction, including BAC clones.

Delivery: TransforMax™ EC100™ Chemically Competent E. coli kit contains ten tubes, each containing 50 µL of cells (enough cells for 10 transformations) and pUC19 control DNA.

TransforMax™ EC100™ Electrocompetent E. coli kit contains ten tubes, each containing 100 µL of cells (enough cells for 20 transformations) and pUC19 control DNA.

Caution: For research use only. Not for human or diagnostic use.

Some Products May Appear Restricted

To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.

If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)

If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.