TransforMax™EC100™ Electrocompetent and Chemically Competent E. coli, Biosearch Technologies

Supplier: Lucigen

Certificates

Supplier: Lucigen

About this item

Clone large plasmids with high efficiency.

Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA
Select convenience with chemically competent format: >5 x 10⁸ cfu/µg pUC19 DNA
Choose electrocompetent format for higher efficiencies: >1 x 10¹⁰ cfu/µg pUC19 DNA
Ideal strain for general cloning, subcloning and plasmid preparation due to endA and recA mutation

The highly versatile TransforMax™ EC100™ E

EC100 competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. They are also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG.

coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction, including BAC clones.

Delivery: TransforMax™ EC100™ Chemically Competent E. coli kit contains ten tubes, each containing 50 µL of cells (enough cells for 10 transformations) and pUC19 control DNA.

TransforMax™ EC100™ Electrocompetent E. coli kit contains ten tubes, each containing 100 µL of cells (enough cells for 20 transformations) and pUC19 control DNA.

Caution: For research use only. Not for human or diagnostic use.

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