Customize your in vitro or in vivo transposomics.

• Create custom transposomes for in vitro or in vivo transposomics
• Easily genereate libraries of mutants for metagenomics, functional genomics and strain engineering applications
• Integrate your selectable marker, promoter or replication origin into plasmid or genomic DNA
• Insert up to 12 kb of sequence - large enough for a biosynthetic pathway or operon

EZ-Tn5™ Transposase is a hyperactive form of Tn5 transposase

A typical EZ-Tn5 transposition reaction requires four components: (1) the EZ-Tn5 Transposase; (2) an EZ-Tn5 Transposon; (3) a target DNA; and (4) the presence of Mg²⁺. The highly random insertion of an EZ-Tn5 Transposon into the target DNA proceeds by a cut-and-paste mechanism, catalyzed by the EZ-Tn5 Transposase, and results in a 9-bp duplication of target DNA sequence immediately adjacent to both ends of the Transposon.

This product is covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).

The highly purified, single-subunit enzyme can be used to randomly insert (transpose or "hop") any EZ-Tn5 Transposon into any target DNA in vitro with an efficiency up to >10⁶ insertion clones per standard reaction. When incubated with an EZ-Tn5 Transposon in the absence of Mg²⁺, a stable EZ-Tn5 Transposome™ complex is formed. The Transposome is so stable that it can be electroporated into living cells. Once in the cell, the Transposome is activated by intracellular Mg²⁺ and the EZ-Tn5 Transposon component is randomly inserted into the host's genomic DNA. The three-dimensional structure of a Transposome complex has been elucidated.