MycoAlert™ Mycoplasma Detection Kits, Lonza
About this item
The MycoAlert™ PLUS Mycoplasma Detection Kit is the next generation Lonza MycoAlert™ Kit. It provides higher light output to allow for use with less sensitive plate luminometers or multifunctional readers and testing of unused media, media supplements or water.
- Convenient & fast
- Simply add two reagents to the culture supernatant, perform two luminescence readings and get your results in less than 20 minutes
- Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
- Suited for tube luminometers, plate luminometers and multifunctional readers
The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells
MycoAlert™ Assay Control Set (available separately)
The MycoAlert™ Assay Control Set includes a lyophilized positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ PLUS Mycoplasma Detection Kit. It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio of <1.
Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
9 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The MycoAlert™ PLUS Mycoplasma Detection Kit is the next generation Lonza MycoAlert™ Kit. It provides higher light output to allow for use with less sensitive plate luminometers or multifunctional readers and testing of unused media, media supplements or water.
- Convenient & fast
- Simply add two reagents to the culture supernatant, perform two luminescence readings and get your results in less than 20 minutes
- Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
- Suited for tube luminometers, plate luminometers and multifunctional readers
The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells
MycoAlert™ Assay Control Set (available separately)
The MycoAlert™ Assay Control Set includes a lyophilized positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ PLUS Mycoplasma Detection Kit. It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio of <1.
Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
Documents
Certificates
Search by Lot Number75860-358
Videos
AT-MycoAlert PLUS_Full Instructions_VWR_170315.pdf Manual Mycoplasma Products for Managing Mycoplasma Contaminations Manual Tips for Mycoplasma free cell culture
Manual
Mycoplasma Products for Managing Mycoplasma Contaminations
Additional Product Details
TDS Bioassay-Compatible Luminometers
Datasheet
VWR Focus Equipments and Instruments
Product Literature
Product Family Options
Product Information
- No. of testsAvailabilityPrice
- Supplier Number: LT07-701Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Supplier Number: LT07-703Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Supplier Number: LT07-705Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Supplier Number: LT07-710Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75870-452MycoAlert™ Mycoplasma Detection KitsSupplier Number: LT07-118Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75870-454MycoAlert™ Mycoplasma Detection KitsSupplier Number: LT07-218Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75870-456MycoAlert™ Mycoplasma Detection KitsSupplier Number: LT07-318Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75870-458MycoAlert™ Mycoplasma Detection KitsSupplier Number: LT07-418Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- 75870-450MycoAlert™ Assay Control SetSupplier Number: LT07-518Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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