For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

• Linear detection range 0.5 to 200 U/L for 15 min reaction
• Can be readily automated for processing thousands of samples per day

Glucose dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems’ non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.