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- Antibody Type:Primary
- Antigen Name:Phosphotyrosine
- Antigen Symbol:Phosphotyrosine
- Clonality:Monoclonal
- Clone:PY20
- Conjugation:FITC (Fluorescein Isothiocyanate)
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b
- Reactivity:
- Western Blot:Yes
- Size:0.5 mg
- Environmentally Preferable:
- Form:Liquid
- Antigen Synonyms:pTyr20
- Storage Buffer:Supplied in PBS/NaN3
- Storage Temperature:2-8°C
- Concentration:0.5 mg/mL
- Shipping Temperature:Ambient Temperature
- Immunogen:Phosphotyrosine conjugated to carrier proteins
- Tested Applications:FLISA
- Cat. No.:100242-988
- Supplier no.:1400-02
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.
Type: Primary
Antigen: Phosphotyrosine
Clonality: Monoclonal
Clone: PY20
Conjugation: FITC (Fluorescein Isothiocyanate)
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: