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- Assay Type:Competitive
- Host:
- Primary antibody reactivity:
- Description:Salivary 17-OH Progesterone ELISA
- Environmentally Preferable:
- Detection Range:5 - 5000 pg/ml
- Application:Fertility/Pregnancy
- Regulatory Status:FDA Regulated; CE Approved
- Cat. No.:75871-388
- Supplier no.:SLV-3140
- No. of tests:96 wells
An enzyme immunoassay for the quantitative measurement of active free 17‑hydroxyprogesterone (17 a-OHP) in saliva.
- FDA cleared tests
- Increased stability and linearity
- Ready-to-use reagents and user-friendly assays
- Measurement of the free (unbound) fraction of steroids
- Excellent correlation to mass spectrometry
The steroid 17-α-Hydroxyprogesterone (17-α-OHP) is produced by both the adrenal cortex and gonads
Late onset adrenal hyperplasia:
More recently, 17-α-OHP concentrations have been utilized in the evaluation of androgenized women where late onset 21-hydroxylase is suspected. This condition is clinically very subtle and since the presentation is the same as classical polycystic ovarian disease, basal plasma 17-α-OHP concentrations, unlike classical congenital adrenal hyperplasia, are normal. The diagnosis is made by administration of an ACTH stimulation test.
The DRG Salivary 17α-OHP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody (rabbit) directed towards an antigenic site on the 17α-OHP molecule. Endogenous 17α-OHP of a patient sample competes with a 17α-OHP-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of 17α-OHP in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of 17α-OHP in the patient sample.
Even though 17-α-OHP has relatively little progestational activity, it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Because Cpd-S is produced by 21-hydroxylation of 17-α-OHP, measurement of 17-α-OHP is a useful indirect indicator of 21-hydroxylase activity. In congenital 21-hydroxylase deficiency, the most common variety of Congenital Adrenal Hyperplasia (CAH), 17-α-OHP is secreted in abundant excess. It is moderately elevated in the 11-β-hydroxylase deficiency as well. Measurement of 17-α-OHP is therefore valuable in the initial diagnosis of CAH.