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Specifications
- Host:
- Primary antibody reactivity:
- Description:Fasciola hepatica IgG ELISA
- Environmentally Preferable:
- Application:Infectious Diseases
- Regulatory Status:FDA Regulated; CE Approved
- Cat. No.:75871-086
- Supplier no.:EIA-4503
- No. of tests:96 wells
Specifications
About this item
An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to Fasciola in serum.
- High quality assays with reproducible and reliable results
- Ready-to-use reagents with internal controls
- Very good precision and sensitivity
- Short assay time and incubation steps at room temperature
- Simple and technician friendly tests
The DRG Fasciola IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Fasciola antigen from adult liver fluke. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Fasciola-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti‑IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Fasciola-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.