An enzyme immunoassay for the quantitative determination of IgG-class antibodies to Tetanus toxin in human serum or plasma.

• High quality assays with reproducible and reliable results
• Ready-to-use reagents with internal controls
• Very good precision and sensitivity
• Short assay time and incubation steps at room temperature
• Simple and technician friendly tests

The DRG Tetanus toxin IgG Enzyme Immunoassay Kit provides materials for the quantitative determination of IgG-class antibodies to Tetanus toxin in human serum or plasma.This assay is intended for in vitro diagnostic use only.Clostridia are anaerobic spore-forming gram-positive bacilli whose pathogenicity depends on the release of highly destructive enzymes or powerful exotoxins

The DRG Tetanus toxin IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Tetanus toxoid antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Tetanus toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti‑IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Tetanus toxin-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

Clostridium tetani is ubiquitously present in the soil and in the faces of various animals and produces (among others) the potent neurotoxin tetanospasmin which is released by autolysis. Hence, tetanus