The ReliaPrep RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes from very small amounts of input material.

• Efficiently Purify RNA from as Few as 100 Cells or 0.25mg Tissue
• Simple, 30-minute protocol
• Elutes in small volume, generating highly concentrated RNA
• No phenol/chloroform or ß-mercaptoethanol required

The ReliaPrep RNA Cell Miniprep System provides a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15ul). Using this membrane-based purification system, from 100 to 5 x 10(6) cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.

The system incorporates a DNase treatment step directly on the minicolumn membrane and removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol: chloroform extractions or ethanol precipitations for pure RNA that does not require additional purification or concentration of the RNA in demanding applications.

With this membrane-based purification system, from 100 to 5 × 10⁶ cultured cells or 0.25 to 20 mg of tissue can be processed per purification.