The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells.

• Purifies Total RNA From 96 Samples in Under an Hour
• Vacuum-based method. No centrifugation required
• Plate design prevents cross-contamination during sample processing
• Designed for both manual and automated RNA purification

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation. Protocols are available for Beckman Coulter and PerkinElmer instruments.

The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20" of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold or similar manifold. The Vac-Man 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol: chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.