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Specifications
- Application:
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:DNA gel bandsAmplified/modified DNA
- Sample type:Gel matrixPCR and other enzymatic reactions
- Target molecule:DNA
- Description:Wizard SV Gel and PCR Clean-Up System, 1,000 preps
- Size:1,000 preps
- Environmentally Preferable:
- Storage Conditions:15°C to 30°C
- Description:Wizard SV Gel and PCR Clean-Up System
- Cat. No.:PAA9285
- Supplier no.:A9285
Specifications
About this item
The Wizard SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100 bp to 10 kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.
- DNA Fragments or PCR Products Can Be Purified in Fifteen Minutes
- Up to 95% Recovery Eluted in as Little as 15 μL
- Purified DNA Routinely Achieves 700 Bases with 98% Accuracy in Automated Fluorescent Sequencing
- DNA Suitable for Automated Fluorescent Sequencing, Cloning, Labeling, Restriction Enzyme Digestion, or in-vitro Transcription/Translation without Further Manipulation
- One System Can Replace up to Four Kits from Other Suppliers
Up to 95% recovery is achieved depending upon the DNA fragment size
The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in-vitro transcription/translation without further manipulation.
PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40 μg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed.