A silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures. The miniprep procedure can be completed in 45 minutes or less.

• 1-20microg High-Quality Plasmid DNA from 1-10ml Cultures
• Process up to 20 minipreps in 45 minutes
• Choice of spin or vacuum purification formats
• No phenol extractions or precipitations required

The Wizard Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1-10ml of overnight E. coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).

The purified plasmid DNA can be used directly for automated fluorescent BigDye terminator DNA sequencing and for other standard molecular biology techniques without further manipulation.

With this system, users can purify Plasmid DNA from one to 10 mL of overnight E.coli culture.