About this item
The ROS-Glo H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions.
- Specific, Direct Detection of Hydrogen Peroxide (H2O2)
- Fast assay involves a simple two-reagent-addition protocol
- Substrate reacts directly with H2O2; no need for HRP as a coupling enzyme
The ROS-Glo H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatized luciferin substrate is incubated with sample and reacts directly with H2O2 to generate a luciferin precursor. Addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo Recombinant Luciferase to produce light signal that is proportional to the level of H2O2 present in the sample.
The addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H2O2 present in the sample.
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Specifications
- Storage Conditions:Store all components at –30°C to –10°C.
- Storage Temperature:-30°C to -10°C
Specifications
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