The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.

• Ultra-Sensitive Detection of Firefly and NanoLuc(R) Luciferase Activities in a Single Sample
• Greater sensitivity at low expression levels
• Choose either firefly or NanoLuc(R) luciferase as primary reporters
• Improved reagent stability brings more flexibility to assay design

The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that enables you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample. The firefly luciferase (Fluc) activity is measured first using ONE-Glo EX Luciferase Assay Reagent. NanoDLR Stop & Glo Reagent is added to quench the firefly signal and provide the furimazine substrate needed to measure Nluc activity. This convenient “add-read-add-read” format generates stable glow-type luminescent signals for both reporters directly from cells with no lysis or cell preconditioning steps required. Potent Fluc inhibition coupled with the high-intensity Nluc luminescence create a dual assay in which both reporters have maximum sensitivity in an assay format that is both easy-to-use and flexible. The NanoDLR workflow is compatible with assays or screens in any plate size, supports batch processing, and is ideal for any luminometer with no specific filter or injector requirements. Excellent signal separation allows for use of either Nluc, Fluc or both as the dynamic experimental reporter. Co-reporter control vectors expressing either Nluc or Fluc from a variety of promoters are available individually or can be obtained in reagent/vector bundles that provide the NanoDLR reagent with the TK or PGK control vector of choice for simple adoption of the NanoDLR Assay.

Potent Fluc inhibition with the high-intensity Nluc luminescence create a dual assay in which both reporters have maximum sensitivity in an assay format that is both easy to use and flexible. The NanoDLR workflow is compatible with assays or screens in any plate size, supports batch processing, and is ideal for any luminometer with no specific filter or injector requirements. Excellent signal separation allows for use of Nluc, Fluc, or both as the dynamic experimental reporter. Co-reporter control vectors expressing Nluc or Fluc from a variety of promoters are available individually or can be obtained in reagent/vector bundles that provide the NanoDLR reagent with the TK or PGK control vector of choice for simple adoption of the NanoDLR Assay. The ONE-Glo EX Luciferase Assay Reagent is also available individually, allowing use of the same firefly luciferase reagent in both single and dual assays. The reconstituted reagent has increased stability at room temperature and 4°C, which simplifies repeat use over long experiments and reducing waste.

NanoDLR Stop and Glo® Reagent is added to quench the firefly signal and provide the furimazine substrate needed to measure Nluc activity. This convenient add-read-add-read format generates stable glow-type luminescent signals for both reporters directly from cells with no lysis and cell preconditioning steps required.