Thermo Scientific SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for picogram to high-femtogram level detection.

• Picogram to Femtogram sensitivity: Detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
• Long signal duration: Incubated blots provide up to 24 hours of usable light output when conditions are optimized
• Stable reagent: Eight hour working solution stability; One-year kit stability at room temperature

SuperSignal West Pico PLUS Substrate provides excellent signal intensity and sensitivity for western blotting with horseradish peroxidase (HRP) enzyme conjugates. The intensity of the light emission combined with the exceptional duration allows for acquiring multiple exposures to effortlessly obtain publication-quality blot images. The chemiluminescent substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

Provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5 K to 1:10 K secondary antibody dilutions of a 1 mg/ml stock solution.

Optimized for dilute antibody concentrations:
0.2-1.0 ug/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)
10-50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)

Optimized for dilute antibody concentrations:
0.2 to 1.0 ug/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)
10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)

The intensity of the light emission combined with the exceptional duration allows for acquiring multiple exposures to effortlessly obtain publication-quality blot images. The chemiluminescent substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.