Middlebrook OADC Enrichment is a supplement recommended for isolation and cultivation of Mycobacteria.

• Enhances recovery on primary isolation
• Dextrose is an energy source
• Catalase destroys toxic peroxides
• Promotes mycobacterial growth

Supplementation of the agar base is required in order to obtain mycobacterial growth. In the enriched medium, sodium chloride maintains osmotic equilibrium; oleic acid, as well as other long chain fatty acids, can be utilized by tubercle bacilli and plays an important role in the metabolism of mycobacteria; the primary effect of albumin is that of protection of the tubercle bacilli against toxic agents and, therefore, it enhances their recovery on primary isolation; dextrose is an energy source; and catalase destroys toxic peroxides that may be present in the medium.