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Specifications
- Description:2X Rapid Ligation Buffer
- Size:3 x 500 µL
- Cat. No.:PAC6711
- Supplier no.:C6711
Specifications
About this item
The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors. Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer.
- Ligation of cohesive ends in five minutes, blunt ends in 15 minutes at room temperature
- Use with 5´, 3´ or blunt-ended DNA inserts
- No additional buffer modifications required
- No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. ligations conducted at room temperature