Exonuclease III

Exonuclease III is a 3' --> 5' exonuclease specific for dsDNA.

For labeling in vitro, sequencing, mapping, mutagenesis, duplex shortening
A 3´→5´ Exonuclease Specific for Double-Stranded DNA
Control deletion rate by varying incubation temperature
Inactivate Exonuclease III by heating at 75°C for 10 minutes
Supplied at a concentration of 150-200u/microl

Exonuclease III is a 3'-->5' exonuclease specific for double-stranded DNA. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3'-OH at nicks, blunt ends, recessed ends and 3'-overhangs of less than 4 bases, yielding nucleoside 5'-phosphates. Exonuclease III will also degrade DNA from 3'-phosphate ends due to its intrinsic 3'-phosphatase activity. In addition, the enzyme has apurinic endonuclease activity and ribonuclease H activity. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments.

Storage Conditions:
–30...–10 °C
Enzyme Name:
Exonuclease III
Concentration:
150 - 200 units/µl
Tested Applications:
Labeling in vitro, Sequencing, Mapping, Mutagenesis, Duplex Shortening