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Specifications
- Concentration:10 U/µl
- Description:T4 RNA Ligase
- Size:500 U
- Cat. No.:PAM1051
- Supplier no.:M1051
Specifications
About this item
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.
- Joins ssRNA or ssDNA onto the 5´-Phosphoryl End of ssRNA or ssDNA
- Adds [5´-32P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA
- Can be inactivated by heating at 65°C for 15 minutes
- Supplied at a concentration of 10 u/microl
- For ligation, Labeling and cloning
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5'-32P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.