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Specifications
- Application:
- Description:T7Select®10-3 Cloning Kit
- Environmentally Preferable:
- Cat. No.:EM70550-3
- Supplier no.:70550-3
Specifications
About this item
Novagen's T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7.
- Attractive display vector
- Easy to grow and replicates more rapidly than either bacteriophage l or filamentous phage
- T7 phage particle is extremely robust, and is stable to harsh conditions
- Uses the T7 capsid protein to display peptides or proteins on the surface of the phage
This system has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage) and peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage).
Phage assembly takes place in the E. coli cytoplasm and mature phage are released by cell lysis. Peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly.