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- Assay duration:Multiple steps
- Assay Type:Competitive
- Conjugate ELISA:Unconjugated
- Format:Pre-coated
- Primary antibody reactivity:RatMouse
- Target protein:Testosterone
- Description:Testosterone Mouse/Rat ELISA
- Size:1 kit
- Sample Type:Serum, Plasma
- Detection Method:Colorimetric
- Detection Range:0.1 - 25 ng/ml
- Storage Temperature:2...8 °C
- Sample Volume:10 µl
- Sensitivity:0.066 ng/ml
- Regulatory Status:RUO
- Cat. No.:101076-640
- Supplier no.:RTC001R
- No. of tests:96 wells
96 wells (1 kit) - ELISA is a competitive enzyme immunoassay for the quantitative measurement of testosterone.
The BioVendor Mouse/Rat Testosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. An unknown amount of testosterone present in the sample and a defined amount of testosterone conjugated to horseradish peroxidase compete for the binding sites of testosterone antiserum coated to the wells of a microplate. After one-hour incubation on a shaker the microplate is washed four times. After addition of the substrate solution the concentration of testosterone is inversely proportional to the optical density measured.