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- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:PEDF
- Description:Human PEDF ELISA
- Size:1 kit
- Sample Type:Serum, Plasma-Citrate
- Detection Method:Colorimetric
- Shelf Life:See expiration date
- Detection Range:0.15 - 6 ng/ml
- Storage Temperature:2...8 °C
- Sample Volume:10 µl
- Sensitivity:0.045ng/ml
- Regulatory Status:RUO
- Cat. No.:102552-522
- Supplier no.:RD191114200R
- No. of tests:96 wells
96 wells (1 kit) - ELISA is a sandwich enzyme immunoassay for the quantitative measurement of PEDF.
In the BioVendor Human PEDF ELISA, standards, quality controls and samples are incubated in microtitration wells coated with polyclonal anti-human PEDF antibody. After 60 min incubation and washing, biotin labelled polyclonal anti-human PEDF antibody is added and incubated with captured PEDF for 60 min. After another washing, streptavidin-HRP conjugate is added. After 60 min incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of PEDF. A standard curve is constructed by plotting absorbance values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.