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Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Human
- Size:0.1 mg
- Storage Conditions:Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for three days
- Endotoxin Content:<0.1 EU/μg
- Reconstitution Instructions:Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non sterile product before using in cell culture.
- Protein Synonyms:HSP90B1|ECGP|Tumor rejection antigen 1|Endothelial cell (HBMEC) glycoprotein|GRP-94|Endoplasmin|94 kDa glucose-regulated protein|GRP94|TRA1|Heat shock protein 90 kDa beta member 1|gp96 homolog
- Protein/Peptide Name:GP96 (HSP90B1) Human HEK293
- Purity:>90%
- Molecular Weight:90.9 kDa (calculated)
- Sequence:ASDDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEETAKESTAEGPKLHHHHHH
- Endotoxin Level:<0.1 EU/μg
- Formulation:Filtered (0.4 μm) and lyophilized in phosphate buffered saline (PBS) pH 8.0, 1% (w/v) Sucrose, 4% (w/v) Mannitol
- Shipping Temperature:On ice
- Tested Applications:Western blotting, ELISA, Cell culture and/or animal studies
- Cat. No.:102830-698
- Supplier no.:RD172379100
Specifications
About this item
Total 789 AA. MW: 90.9 kDa (calculated). UniProtKB acc. No. Q5CAQ5 (Asp22–Glu798). N-terminal linker (2 extra AA), C-terminal linker (4 extra AA) and C-terminal His-tag (6 extra AA). Protein identity confirmed by LC-MS/MS.
- Quality Control Test
- BCA to determine quantity of the protein
- SDS PAGE to determine purity of the protein
- LAL to determine quantity of endotoxin
Sequence: ASDDEVDVDG TVEEDLGKSR EGSRTDDEVV QREEEAIQLD GLNASQIREL REKSEKFAFQ AEVNRMMKLI INSLYKNKEI FLRELISNAS DALDKIRLIS LTDENALSGN EELTVKIKCD KEKNLLHVTD TGVGMTREEL VKNLGTIAKS GTSEFLNKMT EAQEDGQSTS ELIGQFGVGF YSAFLVADKV IVTSKHNNDT QHIWESDSNE FSVIADPRGN TLGRGTTITL VLKEEASDYL ELDTIKNLVK KYSQFINFPI YVWSSKTETV EEPMEEEEAA KEEKEESDDE AAVEEEEEEK KPKTKKVEKT VWDWELMNDI KPIWQRPSKE VEEDEYKAFY KSFSKESDDP MAYIHFTAEG EVTFKSILFV PTSAPRGLFD EYGSKKSDYI KLYVRRVFIT DDFHDMMPKY LNFVKGVVDS DDLPLNVSRE TLQQHKLLKV IRKKLVRKTL DMIKKIADDK YNDTFWKEFG TNIKLGVIED HSNRTRLAKL LRFQSSHHPT DITSLDQYVE RMKEKQDKIY FMAGSSRKEA ESSPFVERLL KKGYEVIYLT EPVDEYCIQA LPEFDGKRFQ NVAKEGVKFD ESEKTKESRE AVEKEFEPLL NWMKDKALKD KIEKAVVSQR LTESPCALVA SQYGWSGNME RIMKAQAYQT GKDISTNYYA SQKKTFEINP RHPLIRDMLR RIKEDEDDKT VLDLAVVLFE TATLRSGYLL PDTKAYGDRI ERMLRLSLNI DPDAKVEEEP EEEPEETAED TTEDTEQDED EEMDVGTDEE ETAKESTAEG PKLHHHHHH
Other names: Endoplasmin; Heat shock protein 90 kDa beta member 1; HSP90B1; 94 kDa glucose-regulated protein; GRP-94; GRP94; Tumor rejection antigen 1; TRA1; gp96 homolog; ECGP; Endothelial cell (HBMEC) glycoprotein