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Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.1 mg
- Storage Conditions:Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week
- Endotoxin Content:<0.1 EU/μg
- Reconstitution Instructions:Add 0.1 M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
- Protein Synonyms:UNQ170/PRO196|ARP2|Angiopoietin-Related Protein 2|ANGPTL2
- Protein/Peptide Name:Angiopoietin-Like Protein 2 Human E. coli
- Purity:>95%
- Molecular Weight:56 kDa (calculated)
- Sequence:MKHHHHHHASQEDGFEGTEEGSPREFIYLNRYKRAGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHLATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIINQISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQALEDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWSGRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVVMMIRPNPNTFH
- Endotoxin Level:<0.1 EU/μg
- Formulation:Filtered (0.4 μm) and lyophilized in 0.5 mg/ml in 0.05 M Acetate buffer pH 4.0
- Shipping Temperature:At ambient temperature
- Tested Applications:Western blotting, ELISA
- Cat. No.:101189-678
- Supplier no.:RD172305100
Specifications
About this item
Total 481 AA. MW: 56 kDa (calculated). UniProtKB acc.no. Q9UKU9 (Gln23-His493). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.
- Quality Control Test
- BCA to determine quantity of the protein
- SDS PAGE to determine purity of the protein
- LAL to determine quantity of endotoxin
Sequence: MKHHHHHHAS QEDGFEGTEE GSPREFIYLN RYKRAGESQD KCTYTFIVPQ QRVTGAICVN SKEPEVLLEN RVHKQELELL NNELLKQKRQ IETLQQLVEV DGGIVSEVKL LRKESRNMNS RVTQLYMQLL HEIIRKRDNA LELSQLENRI LNQTADMLQL ASKYKDLEHK YQHLATLAHN QSEIIAQLEE HCQRVPSARP VPQPPPAAPP RVYQPPTYNR IINQISTNEI QSDQNLKVLP PPLPTMPTLT SLPSSTDKPS GPWRDCLQAL EDGHDTSSIY LVKPENTNRL MQVWCDQRHD PGGWTVIQRR LDGSVNFFRN WETYKQGFGN IDGEYWLGLE NIYWLTNQGN YKLLVTMEDW SGRKVFAEYA SFRLEPESEY YKLRLGRYHG NAGDSFTWHN GKQFTTLDRD HDVYTGNCAH YQKGGWWYNA CAHSNLNGVW YRGGHYRSRY QDGVYWAEFR GGSYSLKKVV MMIRPNPNTF H
Other names: Angiopoietin-Related Protein 2, ANGPTL2, ARP2, UNQ170/PRO196