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Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Size:0.025 mg
- Storage Conditions:Store lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week
- Endotoxin Content:<1.0 EU/µg
- Reconstitution Instructions:Add deionized water to prepare a working stock solution of approximately 0.25 mg/ml and let the lyophilized pellet dissolve completely.
- Protein Synonyms:NEFL|NFL|NF-L|68 kDa neurofilament protein|Neurofilament triplet L protein|NF68
- Protein/Peptide Name:Neurofilament light polypeptide Human E. coli
- Purity:>90%
- Molecular Weight:62.5 kDa (calculated)
- Sequence:MKHHHHHHASSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
- Endotoxin Level:<1.0 EU/µg
- Formulation:Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 15 mM Tris, 85 mM Glycine, pH 8.6
- Shipping Temperature:At ambient temperature
- Tested Applications:Western blotting, ELISA
- Cat. No.:101189-770
- Supplier no.:RD172009100-H
Specifications
About this item
Total 551 AA. MW: 62.5 kDa (calculated). UniProtKB acc.no. P07196 (Ser2-Asp543). N-terminal His-tag (9 extra AA). Protein identity confirmed by LC-MS/MS.
- Quality control test
- BCA to determine quantity of the protein
- SDS PAGE to determine purity of the protein
- LAL to determine quantity of endotoxin