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Specifications
- Enzyme type:Recombinant
- Source:E. coli
- Species:E. coli
- Size:0.05 mg
- Tag sequence:N-6 His tag
- Storage Conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Endotoxin Content:<0.1 ng/ug (1 IEU/ug) as determined by LAL test.
- Enzyme Name:beta-Glucuronidase
- Enzyme Synonyms:Beta-glucuronidase, uidA, GUS, Beta-GUS, GUSB
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular Weight:69.9 kD
- Sequence:Met1-Gln603
- Formulation:Supplied as a 0.2 µm filtered solution of PBS,pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested Applications:Biological assays
- Cat. No.:75791-186
- Supplier no.:92-300
Specifications
About this item
Beta-D-glucuronidase from E
This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: N-6 His tag
coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl- beta -d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.