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Human Recombinant UBE2S (from E. coli)
Human Recombinant UBE2S (from E. coli)
Catalog # 75789-214
Supplier:  Prosci
CAS Number:  
Human Recombinant UBE2S (from E. coli)
Catalog # 75789-214
Supplier:  Prosci
Supplier Number:  91-269
CAS Number:  

Specifications

  • Protein/Peptide Type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Size:
    0.05 mg
  • Tag sequence:
    N-GST tag
  • Storage Conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
  • Endotoxin Content:
    <0.1 ng/ug (1 IEU/ug) as determined by LAL test.
  • Protein Synonyms:
    Ubiquitin-Conjugating Enzyme E2-24 kDa|E2-EPF|Ubiquitin-Conjugating Enzyme E2 S|E2EPF|UBE2S|Ubiquitin-Conjugating Enzyme E2-EPF5|Ubiquitin Carrier Protein S|Ubiquitin-Protein Ligase S
  • Protein/Peptide Name:
    UBE2S
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular Weight:
    50.13 kD
  • Sequence:
    Met1-Leu222
  • Formulation:
    Supplied as a 0.2 um filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested Applications:
    Bioassay
  • Cat. No.:
    75789-214
  • Supplier no.:
    91-269

Specifications

About this item

Ubiquitin-Conjugating Enzyme E2 S (UBE2S) is a member of the Ubiquitin-Conjugating Enzyme family. UBE2S interacts with CDC20, FZR1/CDH1 and VHL. UBE2S can form a thiol ester linkage with Ubiquitin in an Ubiquitin Activating Enzyme-Dependent manner, a characteristic property of Ubiquitin Carrier Proteins. UBE2S acts as an essential factor of the Anaphase Promoting Complex/Cyclosome, a cell cycle-regulated Ubiquitin ligase that controls progression through mitosis. UBE2S is also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A.

  • Fusion-Tag: N-GST tag

This recombinant protein can be used for biological assays. For research use only.