Mix-n-Stain™ Enzyme Antibody Labeling Kits allow you to easily conjugate up to 100 µg of your antibody to a detection enzyme with minimal hands-on time and no purification.

• Label 5 to 20 µg, 20 to 50 µg, or 50 to 100 µg Ab
• Choice of HRP, AP, or glucose oxidase
• Labeling takes a few hours with minimal hands-on time
• No purification, 100% recovery

Mix-n-Stain™ Enzyme Antibody Labeling Kits are a revolutionary antibody labeling technology that allows you to covalently conjugate up to 100 µg of your antibody with a detection enzyme with minimal hands-on time and no purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers.

Horseradish peroxidase (HRP) conjugates can be used for Western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The enzyme label can be visualized with chromogenic substrates such as DAB, ABTS, TMB and TMBUS in the presence of hydrogen peroxide. Label 5 to 20 µg, 25 to 50 µg, or 50 to 100 µg of antibody in about 3 hours

Glucose oxidase (GOx) is an enzyme which catalyses the oxidation of glucose with the release of hydrogen peroxide. Label 25 to 50 µg, or 50 to 100 µg of antibody in 2 hours.

Alkaline phosphatase (AP) conjugates can be used for ELISA, immunohistochemistry and other standard immunoassay applications. The enzyme label can be visualized with chromogenic substrates such as BCIP and PNPP as well as fluorogenic substrate such as MUP. Label 25 to 50 µg, or 50 to 100 µg of antibody in 2 hours.

Biotium also offers Mix-n-Stain™ Kits for labeling antibodies, nanobodies, or small ligands with bright & photostable CF® dyes and other labels.