Lab Vision™ Tris Buffered Saline and Tween 20 (20X), Thermo Scientific
Catalog # 10129-558
Supplier: Epredia
CAS Number:
Specifications
- Description:Tris Buffered Saline and Tween 20 (20X)
- Size:1000 mL
- Cat. No.:10129-558
- Supplier no.:TA-999-TT
Specifications
About this item
Utilize this specially formulated reagent as a wash buffer for immunolabeling techniques.
- For in vitro diagnostic use
- Tween 20 is added to promote more effective washings, resulting in decreased nonspecific background staining
- Tween 20 aids reagent spreading on slides
Enzyme is Alkaline Phosphatase.
Ordering information: For in vitro diagnostic use.
Diluting Procedure: To make 1X TBS/Tween 20 solution dilute conctentrate 20X with distilled water. Use 50 mL PBS concentrate per 950 mL DI water. Measure and pour appropriate volume of 20X PBS concentrate into a mixing flask and add DI water to final volume. Stir briefly. The 1X solution should be pH 7.6 ±0.2. The pH of the soution may require slight adjustment. Adjust pH with 1N HCl (if pH is high) or 1N NaOH (if pH is low).
Diluting Procedure: To make 1X TBS/Tween 20 solution dilute conctentrate 20X with distilled water. Use 50 mL PBS concentrate per 950 mL DI water. Measure and pour appropriate volume of 20X PBS concentrate into a mixing flask and add DI water to final volume. Stir briefly. The 1X solution should be pH 7.6 ±0.2. The pH of the soution may require slight adjustment. Adjust pH with 1N HCl (if pH is high) or 1N NaOH (if pH is low).
Caution: Store at Room Temperature. Stable for 24 months from date of manufacture. Use 1X diluted solution within one week.