Cell Biolabs' Total Bile Acid Assay Kits provide a convenient 96-well plate-based method to measure the total bile acid content in a variety of sample types

The reaction used with the colorimetric kit requires the presence of NADH, and thio-NAD+. The thio-NAD+ is reduced to thio-NADH which is detected by colorimetric absorbance. The fluorometric kit requires incubation with NAD+, which is converted to NADH. Diaphorase then uses NADH to reduce resazurin to resorufin, which is detected fluormetrically at 560nm excitation and 590nm emission.

These assays are based on an enzyme driven reaction in which bile acids are incubated in the presence of 3-alpha hydroxysteroiddehydrogenase. Measure total bile acid content as low as 1 µM.