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- Assay Type:Competitive
- Conjugate ELISA:HRP
- Description:s-Glutathione Adduct Competitive ELISA Kit
- Size:96 Assays
- Detection Method:Colorimetric
- Storage Temperature:4°C and –20°C
- Cat. No.:102965-284
- Supplier no.:STA-814
OxiSelect™ s-Glutathione Adduct Competitive ELISA Kit quantifies the glutathionylation of proteins in a standard 96-well plate
Protein standards and unknown samples are added to a plate coated with an s-glutathione protein conjugate. A primary anti-s-glutathione antibody is added and competes for binding between the s-glutathione adduct in the sample and the s-glutathione adduct bound to the plate. High concentrations of s-glutathione adduct in samples leave little antibody to bind to the plate, resulting in a low OD. When little to no s-glutathione adduct appears in samples, most antibody binds to the plate resulting in a high signal.