CytoSelect™ Cell Invasion Assays are ideal for determining the invasive properties of cells

With the CytoSelect™ Cell Invasion Assays, no manual cell counting is required. Quantitation of cells is performed using a colorimetric or fluorometric dye in a 96-well plate reader. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines. Kits are available in 24-well and 96-well formats.

These kits use a Boyden Chamber in which inserts containing a membrane with a defined pore size are nested in the wells of a cell culture plate. The top of the insert membrane is coated with a thin layer of a basement membrane protein matrix. Invasive cells are seeded above the membrane and must degrade the protein matrix layer before migrating toward a chemoattractant in the lower chamber.