CytoSelect™ 24-Well Haptotaxis Assays are ideal for determining the haptotactic properties of cells

With the CytoSelect™ Haptotaxis Assays, no manual cell counting is required. Quantitation of cells is performed using a colorimetric or fluorometric dye in a 96-well plate reader. The 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines.

These kits use a Boyden Chamber in which inserts containing a membrane with a defined pore size are nested in the wells of a cell culture plate. The underside of each insert is coated with an ECM protein (collagen or fibronectin). Cells are seeded above the membrane and are allowed to migrate toward a chemoattractant in the lower chamber.