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Specifications
- Description:Cellular Antioxidant Assay Kit
- Size:192 Assays
- Storage Temperature:4°C and –20°C
- Cat. No.:102964-976
- Method:Fluorometric
- Supplier no.:STA-349
Specifications
About this item
The OxiSelect™ Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within a cellular environment. Adherent cells are first cultured in a 96-well black fluorescence cell culture plate until confluent
Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.