Dispase is a mild protease which cleaves fibronectin, collagen IV and to a lesser extent collagen I.

Lyophilized and partially purified dispase is used for Neutral Protease (Dispase) Assay, tissue disaggregation and subcultivation, prevention of unwanted cell clumping, preparation of cells for culture, separation of intact epidermis from dermis and intact epithelial sheet in culture from the substratum, harvest and transfer of cell lines, gentle and intact detachment of epidermal cells.