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Specifications
- Environmentally Preferable:
- Storage Conditions:-20°C
- Species Reactivity:Mammalian samples
- Sample Type:Partially purified and recombinant enzyme
- Shelf Life:12 Months
- Cat. No.:10013-762
- No. of assays/kit:100 assays
- Supplier no.:K588-100
Specifications
About this item
Simple assay to measure activity of purified enzyme and screening inhibitors/activators of HMGR
- Detection method- Absorbance (OD 340 nm)
- Applications- Measurement of HMG-CoA reductase activity
- - Screening/studying/characterizing HMG-CoA reductase inhibitors/activators
HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol. BioVision’s HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.