The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).

• Quantitative and stable
• Achieve labeling efficiency greater than 90% in one hour
• 20-fold increase in signal-to-noise compared to unlabeled native glycan MS analysis
• Able to identify and characterize up to six samples concurrently
• Procedure and reagents optimized for excellent labeling efficiency and recovery of glycans

Native glycans are difficult to study by mass spectrometry because of their poor ionization efficiency. Quantitation of glycans is particularly challenging due to the lack of standards for all naturally-occurring glycans and difficulties reproducibly quantifying multiple samples. The aminoxy group has better reactivity with carbonyls and better stability of the labeled product compared to hydrazides. Labeling with the aminoxyTMT reagents improves ionization of glycans, thus improving sensitivity, and enables relative quantitation of glycans for up to six samples concurrently.