HisPur™ Cobalt spin plates are convenient, ready-to-use, pre-dispensed filter plates for the efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells

• High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
• Specificity – cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
• Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
• Versatility – purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
• Cost effective – resin can be reused several times or discarded after single use
• Flexibility – available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

These spin plates are ideal for simultaneous processing of multiple small (1 mg/well binding capacity) samples. The spin plates contain a tetradentate chelating agarose resin charged with divalent cobalt (Co²⁺) for obtaining high-purity his-tagged proteins. This resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole).

These spin plates are ideal for simultaneous processing of multiple small (1 mg/well binding capacity) samples. Store at 4˚C.