Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

• Can be used in tandem with trypsin to decrease tryptic missed cleavages thus enhancing digestion
• Better protein characterization results from overlapping peptides with complementary chromatographic, ionisation and fragmentation properties thereby increasing sequence coverage
• Effective enzyme activity under highly denaturing conditions
• C-terminal lysine cleavage specificity of 90% for a complex protein sample
• Stable since it is provided in a lyophilized format

This Lys-C is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes

Applications include improved sequence coverage of protein digests, de novo sequencing, epigenetic studies and in-gel and in-solution digestion of proteins.

Lys-C has high activity and specificity for lysine residues resulting in larger peptides and less sample complexity than trypsin (i.e. fewer peptides). Unlike trypsin, Lys-C can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. These unique Lys-C properties ensure high digestion efficiency when used alone or followed by tryptic digestion.