About this item
Pierce™ HisPur™ Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.
- High capacity binding up to 60 mg of 6×His-tagged protein per milliliter of resin
- Purify proteins using native or denaturing conditions
- Resin can be reused at least five times without reduction in performance
- Purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates
The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni²⁺). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.