Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,
yeast or bacteria.

• Convenient
• No proprietary ingredients—fully disclosed formulation
• Multiple package sizes
• Regular formulation—includes a separate vial of EDTA solution for optional metalloprotease inhibition
• Lysis buffer compatible

The cocktail inhibits serine and cystine proteases, calpain proteases, and metalloproteases

Proteases make up a large category of enzymes, including endopeptidases and exopeptidases, which catalyze the hydrolytic breakdown of proteins into peptides or amino acids in a wide variety of cell and tissue types. Proteases are regulated and compartmentalized in living cells, but cell lysis releases and mixes them with other extracted proteins. Their activity can thus lead to the loss of a large number of valuable proteins, adversely affecting downstream applications. For this reason, it is common practice to spike cell lysis buffers or cell extracts with a cocktails of compounds known to inactivate or inhibit proteases. Halt Protease Inhibitor Cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.

The unique EDTA-Free formulation effectively inhibits most metalloprotease activity while allowing optimal action of nuclease activity for removing nucleic acids from samples.