Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein L is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of mouse and human antibodies in immunoassays and antibody purification protocols.

• Contains four immunoglobulin-binding domains per protein
• Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
• Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Form: salt-free powder
A280 of 0.1% solution: 0.80

Isoelectric point (pI): 4.8