Thermo Scientific Pierce Substrate Buffer include optimized, reliable and convenient stock solutions for HRP and alkaline phosphatase assays detection respectively.

• Ready-to-use
• Reliable and convenient
• Minimize assay-to-assay variability
• Store product at 4˚C
• Shipped at ambient temperature

Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP

Buffered stable hydrogen peroxide (H2O2) is used by researchers who prefer to make their own HRP-ELISA substrates. This is easily done by adding a chromogen of choice to the buffer. Stable Peroxide Substrate Buffer has a long shelf life (12 months after receipt) and is provided as a 10-fold concentrate. A total volume of 1000 mL can be made from one 100 mL bottle of concentrate. If you use 10 mL of substrate per plate, this will allow you to make substrate for 100 plates from only one bottle of Stable Peroxide Substrate Buffer.

Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.