AminoLink™ Plus Coupling Resin allows simple and efficient covalent immobilization of proteins to a beaded agarose support, providing a valuable tool for affinity purification of antibodies, antigens or other biomolecules.

• Aldehyde-activated crosslinked 4% beaded agarose
• Ideal for antibodies and other proteins, immobilize molecules via primary amines
• Efficient >85% coupling over a wide range of pH (4 - 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
• Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
• Prepared affinity resin is adaptable to column and batch affinity techniques and is reusable for typical applications

AminoLink Plus Resin is beaded agarose support that has been modified to contain aldehyde groups. Antibodies, other proteins, or any molecules with primary amines can be conjugated to the resin by an optimized form of reductive amination. Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.

The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride).