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Specifications
- Description:Immobilized Papain (agarose resin)
- Size:5 mL
- Storage Temperature:+4°C
- Cat. No.:PI20341
- Supplier no.:20341
Specifications
About this item
Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.
- Papain can also be used to generate F(ab')2 fragments
- Immobilized papain canbe substituted fro free papain because it eliminates autolysis and contamination of a sample with the protease
- Allows control of the digestion by removing the pepsin or controlling the flow of sample over an immobilized papain column
- Immobilized papain is more stable against heat-induced denaturation, resulting in longer maintenance of activity
Papain is a nonspecific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilized Protein A or by ion exchange chromatography.