Pierce™ Renilla Luciferase Flash Assay Kit, Thermo Scientific

The Thermo Scientific Pierce Renilla luciferase flash assay kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

Greater sensitivity allows utilization of smaller numbers of cells
Highest sensitivity assays result in decreased reagent consumption
Assays require minimal sample handling
Amenable to automation
Contains a universal cell lysis buffer and optimized flash assay reagent
Allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates.

The protein provides extremely bright flash signal that decays rapidly. The Pierce Renilla Flash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. The kit also is compatible for flash assays with other Renilla luciferases and derivatives that use coelenterazine as a substrate.

When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.

: Kit contains Renilla flash assay buffer(5 or 50 ml) (store at 4 ˚C), 100X Coelenterazine (50 or 500 µl) (store at –80 ˚C) and 2X cell lysis buffer (6 or 60 ml) store at room temperature.

: For research use only. Not for use in diagnostic procedures.

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