Pierce™ Red Firefly Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

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Supplier: Invitrogen

About this item

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

Intracellular Red Firefly luciferase gene, optimised for high expression in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells respectively
High-copy pUC bacterial DNA replication origin

pMCS-Red Firefly Luc is a multiple cloning site plasmid designed to accept a promoter sequence for the study of gene regulation using the intracellular red firefly luciferase reporter with excellent light intensity.
pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalisation.
pTK-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalisation.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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